o:1204 Uticaj sastojaka energetskih napitaka na funkciju miokarda i oksidativni stres u izolovanom srcu i krvi pacova The effect of energy drink ingredients on myocardial function and oxidative stressin isolated heart and blood of rats : doctoral dissertation sr Introduction. Energy drinks (EDs) are widely used by athletes as ergogenic agents. The aims of this study were to determine the acute, chronic and direct effects of Red Bull®, one of the most consumed EDs, on cardiodynamics and the parameters of oxidative stress in physically active and physically inactive rats. Material and method. The study lasted 4 weeks. Rats were divided into 2 groups depending on the consumption of Red Bull (3.75 ml/kg), and each group was divided into 2 subgroups, depending on the physical activity. The trained rats were subjected to a swimming practice (1h per day, 5 days a week). After sacrificing, the animals were sampled for venous blood to determine the plasma redox status. Subsequently, the hearts were isolated and perfused by Langendorf method of retrograde perfusion. The coronary flow and parameters of left ventricular function were monitored: maximum rate of change of left ventricle pressure, minimum rate of change of left ventricle pressure, systolic left ventricular pressure, diastolic left ventricular pressure, and heart rate. The biomarkers of oxidative stress in the blood and effluent were determined by the spectrophotometric methods: superoxide anion radical, hydrogen peroxide, index of lipid peroxidation, nitrite monoxide in the form of nitrite, as well as the parameters of antioxidant protection system: superoxide dismutase, catalase and reduced glutathione.Results. The negative effects of the acute, chronic and direct administration of the ED on cardiac function were registered in untrained animals. In trained animals, the acute and chronic consumption of the ED resulted in increasing the coronary flow and most cardiodynamic parameters, while the direct administration of the ED resulted in decreasing rates of contractility and relaxation, and was associated with the depression of cardiac function. In untrained animals, the administration of the ED had a pro-oxidative effect in the coronary effluent (acute and direct administration) as well as in blood (acute and chronic administration). In trained animals, the acute, chronic and direct administration of the ED had a pro-oxidative potential in the coronary effluent, whereas antioxidant activity was registered in the blood after the acute administration of the ED. Conclusion. Our study contributes to the knowledge on the effects of EDs on cardiodynamics and the redox status correlated with the exercise status. Uvod. Energetska pića (EP-a) se koriste u velikoj meri kao ergogena sredstva. Ciljevi ovog istraživanja bili su da se utvrde akutni, hronični i direktni efekti Red Bull®-a, jednog od EP-a koje se najviše konzumira, na kardiodinamiku i parametre oksidativnog stresa kod fizički aktivnih i fizički neaktivnih pacova. Materijal i metod. Studija je trajala 4 nedelje. Pacovi su podeljeni u 2 grupe u zavisnosti od konzumacije Red Bull-a (3,75 ml/kg), a svaka grupa podeljena je u 2 podgrupe, u zavisnosti od fizičke aktivnosti. Trenirani pacovi su bili podvrgnuti treningu plivanja (1 čas dnevno, 5 dana u nedelji). Nakon žrtvovanja, životinjama je uzet uzorak venske krvi, radi određivanja redoks statusa u plazmi. Nakon toga, srca su izolovana i perfundovana metodom retrogradne perfuzije po Langendorfu. Praćeni su koronarni protok i parametri funkcije leve komore: maksimalna stopa promene pritiska u levoj komori, minimalna stopa promene pritiska u levoj komori, sistolni pritisak leve komore, dijastolni pritisak leve komore i srčana frekvenca. U uzorcima krvi i u efluentu spektrofotometrijskim metodama određivani su biomarkeri oksidativnog stresa: superoksid anjon radikal, vodonik peroksid, indeks lipidne peroksidacije, azot monoksid u formi nitrita, kao i parametri antioksidativnog sistema zaštite: superoksid dismutaza, katalaza i redukovani glutation.Rezultati. Kod netreniranih životinja, zapažen je negativan uticaj akutne, hronične i direktne primene EP-a na funkciju srca. Kod treniranih životinja, akutna i hronična konzumacija EP-a imala je za posledicu povećanje koronarnog protoka i većine kardiodinamskih parametara, dok je direktna administracija EP-a uticala na smanjenje stope kontraktilnosti i relaksacije, odnosno bila je povezana sa depresijom srčane funkcije. Kod netreniranih životinja, primena EP-a ostvarila je prooksidativni uticaj u koronarnom efluentu (akutna i direktna primena), kao i u krvi (akutna i hronična primena). Kod treniranih životinja, akutna, hronična i direktna primena EP-a ispoljili su prooksidativni potencijal u koronarnom efluentu, dok je u krvi nakon akutne primene EP-a zapažen antioksidativni uticaj. Zaključak. Naša studija doprinosi saznanjima o efektima EP-a na kardiodinamiku i redoks status u korelaciji sa trenažnim statusom. ID=525460885 ; D-3306 91552101 7116 91552100 525460885 2020-01-17T13:19:47.053Z 45 no 46 mentor Irena, 1983- 33566567 Pušica 2019 63 mentor Dušica, 1982- 11532647 Đorđević 2019 63 predsednik komisije Vladimir, 1984- 12116071 Živković 2019 63 član komisije Vladimir, 1973- 11117927 Zdravković 2019 63 član komisije Dragan, 1975- 8357735 Radovanović 2019 210 listova 25762271 http://phaidrabg.bg.ac.rs/o:1204 no yes 3 2020-01-17T13:19:47.320Z 70 energetska pića, kardiovaskularni sistem,oksidativni stres, pacovi, plivanje energy drinks, cardiovascular system, oxidative stress, rats,swimming 616.12:613.292(043.3) Kardiovaskularni sistem - Energetska pića 1738 34A05 2019