Efekti primene ekstrakta biljke Satureja hortensis L. na bihevioralne manifestacije neurotoksičnosti izazvane cisplatinom kod pacova Kumburović, Igor, 1975-, 59653385 Uvod: U ovoj studiji su ispitivani efekti ekstrakta biljke Satureja hortensis L. (SHL) na biheviorlane manifestacije neurotoksičnosti izazvane cisplatinom kod pacova. Materijal i metode: Studija je sprovedena na 35 pacova Wistar albino soja, starosti 2 meseca, telesne mase 200-250 g, podeljenih u 7 grupa: kontrolna, cisplatina, cisplatina+ SHL 50 mg/kg, cisplatina+SHL 100 mg/kg, cisplatina+SHL 200 mg/kg, cisplatina+silimarin 100mg/kg i SHL 200 mg/kg. Cisplatina je primenjivana u pojedinačnoj dozi (7.5 mg/kg), dok je antioksidantna suplementacija sprovođena oralno tokom 10 dana. Bihevioralni testovi su izvođeni desetog dana protokola, nakon čega su životinje žrtvovane u cilju dobijanja uzoraka hipokampusa za određivanje markera oksidativnog stresa i apoptoze. Rezultati: Rezultati bihevioralnog ispitivanja su pokazali anksiogeni efekat cisplatine koji je bio ublažen primenom SHL ekstrakta (100 mg/kg) pri čemu je slabiji odgovor bio u grupama tretiranim ekstraktom SHL u dozama od 50 i 200 mg/kg. Takođe, smanjenje neurotoksičnosti izazvano cisplatinom, u smislu povećanja markera oksidativnog stresa i apoptoze, je ostvareno primenom ekstrakta u dozi od 100 mg/kg. Neuroprotektivni efekat SHL je bio sličan dejstvu silimarina u istoj dozi. Ekstrakt SHL nije uzrokovao značajne promene ispitivanih parametara kada je primenjivan samostalno. Zaključak: Anksiogeni efekat, kao manifestacija neurotoksičnosti izazvane cisplatinom, je u bihevioralnim testovima bio uspešno umanjen oralnom primenom ekstrakta SHL u optimalnoj dozi (100 mg/kg). Korisni efekti ove vrste antioksidantne suplementacije su takođe potvrđeni smanjenjem oksidativnog oštećenja i apoptotskih mehanizama. Introduction: In this study we evaluated effects of Saturejа hortensis L. (SHL) extract on behavioral manifestations of cisplatin-induced neurotoxicity in rats. Material and methods: The study was performed on 35 Wistar albino rats, 2 months old, weighting 200-250 g, divided into 7 groups: control, cisplatin, cisplatin+SHL 50 mg/kg, cisplatin+SHL 100 mg/kg, cisplatin+SHL 200 mg/kg, cisplatin+silymarin 100 mg/kg, and SHL 200 mg/kg. Cisplatin was administered in a single dose of 7.5 mg/kg and antioxidant supplementation was performed orally for 10 days. Behavioral testing was performed on 10th day and then the animals were sacrificed in order to obtain the samples for oxidative stress and apoptotic markers in hippocampus. Results: The results of behavioral testing showed cisplatin-induced anxiogenic effect, that was diminished with SHL extract in a dose of 100mg/kg, with much weaker responses to SHL extracts in doses of 50 and 200 mg/kg. Also, the attenuation of cisplatin-induced neurotoxicity, by means of increased oxidative stress and apoptotic markers, was successfully achieved with SHL extract in a dose of 100 mg/kg. The neuroprotective effect of SHL was similar to silymarin action in the same dose. When applied alone, SHL extract induced no significant effect in any of parameters followed. Conclusion: Cisplatin-induced neurotoxicity manifested by anxiogenic response in behavioral testing can be successfully attenuated by oral administration of SHL extract in an optimal dose (100 mg/kg). The beneficial effects of this kind of antioxidant supplementation were also confirmed by decreased oxidative damage and apoptotic mechanisms. Rosić, Gvozden, 1964-, 13578087 Jakovljević, Vladimir, 1971-, 13564775 Ilić, Tihomir, 1966-, 13762919 Selaković, Dragica, 1984-, 14800999 2020 2020 2020 2020 2020 2020 baccalaureate Dissertation 154 lista 12741965 bytes o:1290 ID=24565513 ; D-3371 thesis:7763 cobiss:24565513 https://phaidrakg.kg.ac.rs/o:1290 Thesis:7763 Cobiss:24565513 srp CC BY-NC-ND 2.0 AT http://creativecommons.org/licenses/by-nc-nd/2.0/at/