Karakterizacija i ispitivanje antitumorske aktivnosti kompleksa cinka (II) sa S-alkenil derivatima tiosalicilne kiseline Popović, Ana M., 1979-, 62355209 Dva kompleksa cinka(II) i S-alkenil derivata tiosalicilne kiseline (engl. S-alkenyl derivatives of thiosalicylic acid- SADTA) (ligandi) su sintetisani i karakterisani na osnovu rezultata elementalne mikroanalize, IR, 1H i 13C NMR spektroskopije. Kompleksi su dobijeni direktnom reakcijom ZnCl2 i SADTA, u vodenom rastvoru. Prema fizičkohemijskim i spektroskopskim podacima, zaključujemo da su ligandi bidentatno koordinovani za jon cinka(II). Interakcija kompleksa sa DNA goveđeg timusa (CT-DNA) ispitivana je Uv-vis i fluorimetrijskom metodom. Citotoksična aktivnost kompleksa poređena je sa oksaliplatinom i cisplatinom i analizirana je na dvije različite tumorske ćelijske linije: mišji karcinom debelog crijeva (CT26) i mišji melanom (B16F1), dok su ne-tumorske mišje mezenhimske matične ćelije (mmMSCs) korišćene kao kontrola. Oba kompleksa pokazala su umjerenu aktivnost protiv ćelija mišjeg raka debelog crijeva i melanoma. Smanjenje vijabilnosti ćelija melanoma je izazvano zadržavanjem ćelije u G2 fazi ćelijskog ciklusa i apoptozom. Cink(II) kompleks sa SADTA, iako ima značajno slabiji citotoksični efekat na tumorske ćelije nego oksaliplatina i cisplatina, djeluje selektivnije na tumorske ćelije od poznatih ljekova. In this study, we generated and analized two complexes of zinc (II) and S-alkenyl derivatives of thiosalicylic acid- SADTА (ligands), by microanalysis, IR, H and C NMR spectroscopy. Synthesis of the complexes was accomplished by direct contact of ZnCl2 and SADTА. Physicochemical as well as spectroscopic analysis revealed bidentately coordination of the ligands to the zinc(II) center. Absorption as well as ethidium bromide displacement analyses were used for CTDNA test. Citotoxicity of the complexes was tested in colon carcinoma (CT26) and melanoma (B16F1), as well as in mesenchymal stem cells (mMSCs), all derived from mouse. Citotoxicity of complexes was compared with oxaliplatin and cisplatin. We revealed moderate citotoxicity of complexes against tumor cells. This phenomenon was acompained by induction of apoptosis and G2 phase arrest. The tested zinc (II) complexes has a weaker cytotoxicity than oxaliplatin and cisplatin. However, they show a much more selective effect on tumors than known drugs. Jovanović, Ivan, 1977-, 3999847 Milovanović, Marija, 1979-, 6451047 Radić, Gordana, 1980-, 25784423 Vojvodić, Danilo, 1963-, 10764647 2020 2020 2020 2020 2020 2020 baccalaureate Dissertation 76 listova 2313152 bytes o:1305 ID=28560905 ; D-3389 thesis:7831 cobiss:28560905 https://phaidrakg.kg.ac.rs/o:1305 Thesis:7831 Cobiss:28560905 srp CC BY-ND 2.0 AT http://creativecommons.org/licenses/by-nd/2.0/at/